why is a buccal swab used to collect dnawhy is a buccal swab used to collect dna

Many people are looking for a way to test without one or more persons knowing. Note: If you are using the ChargeSwitchgDNA Normalized Buccal Cell Kit (Cat. No. Do not reuse the beads. This information explains how to collect a DNA sample using a cheek swab. You get too intense too quickly If youre launching yourself at your partner, expecting an intense make-out session right away, youre probably going to be labeled a bad kisser, said sex expert Antonia Hall. Buccal swabs are taken from the back of the mouth and used to collect samples of the saliva and bacteria that live in the mouth. Open the OmniSwab package from the opposite end of the swab tip. However, some collection applications require the use of two swabs at once. Without removing the tube from the MagnaRack, carefully remove the supernatant and discard. Buccal cell collection with a swab is one of the fastest and most common ways for forensic pathologists to establish or refute a link between a suspect and a crime scene. Stay informed about industry news, current events, and the unique properties of Puritan's wide range of products that set them apart from the rest. Please do not write your name or any personal information on this feedback form. The DNA quality was assessed by resolving on agarose gels, checking purity (A260/A280) and finally by microarray hybridization. Nos. For best results, always use a volume of ChargeSwitchElution Buffer (E5) that is equal to or greater than the volume of ChargeSwitch Magnetic Beads used in the protocol. Alhaddad H, Maraqa T, Alabdulghafour S, Alaskar H, Alaqeely R, Almathen F, Alhajeri BH. Cancer Epidemiol Biomarkers Prev 10:687-696, 2001). To release the sample, simply elute the swab into a buffer of your choice. Copyright 2023. Place the sample in the MagnaRack for 1 minute or until the beads have formed a tight pellet. 16. Shake rapidly for 1-2 minutes to completely disperse the beads within the solution. Genetics. It might surprise you to know that much confusion surrounds the real source of genomic DNA in saliva. Call us at 833-347-1665 to make an appointment. Rinse, Swab or Spit -- What's the real source of DNA in saliva? 11020-10), see Automated Extraction Normalized DNA Buccal KitThe ChargeSwitch Magnetic Beads supplied in the ChargeSwitchgDNA Buccal Cell Kit differ in concentration from those supplied in the ChargeSwitchgDNA Normalized Buccal Cell Kit. For the most accurate results, it is critical that researchers select a DNA swab that reduces the risk of contamination from other sources of DNA or RNA, including the swab material itself. Alternatively, TE Buffer, pH 8.5-9.0 is acceptable. 6" sterile DNA-free standard flock swab with plastic handle and tube is a popular choice in DNA collection for forensic applications. Make sure that the beads are in solution at all times and do not become dried. Process samples immediately after collection or store the sample at 4C. What Is A Buccal Swab Used For | Teesnature When removing supernatant, leave samples on the 96-Well Magnetic Separator and aspirate slowly to ensure that the pellet of beads is not disturbed. This makes it easy to collect samples regardless of age, gender, or health status. Contact one of our knowledgeableproduct specialiststoday. Samples can be stored for up to 2 weeks at 4C before processing without a noticeable loss in DNA yield or quality. Want to know how? Its important that you dont touch the end of the swab stick so you dont spread any of your DNA there. Vortex the tube containing the ChargeSwitch Magnetic Beads to fully resuspend and evenly distribute the beads in the storage buffer. Superfecundation is the fertilization of two or more ova from the same cycle by sperm from separate acts of sexual intercourse, which can lead to twin babies from two separate biological fathers. The health care provider will gently scrape the inside of the cheek with a small spatula to collect cells for testing. Collecting a Buccal Swab An Art or a Cinch? The quality of DNA achieved from this diagnostic is ideal for many downstream applications. Move samples to the 96-Well Magnetic Separator. Do not use water for elution. When we kiss, were essentially sharing a tiny bit of our ecosystems were swapping genetic information that our bodies unconsciously process and in doing so, we learn more about each other in an instant than we ever could consciously. If the volume of ChargeSwitchElution Buffer (E5) is lower than the volume of beads used, DNA elution is incomplete. We recommend using the 96 x 2 ml glass-filled polypropylene Unifilter Microplate from Whatman (Cat. Buccal Swabs are cotton-tipped applicators (similar to a Q-Tip) that are used to collect cheek cells from the inside of a persons mouth. Epub 2023 Feb 27. 3. Label the swab with two patient identifiers: the patient's full name and date of birth or medical record number. Use only the ChargeSwitch Magnetic Beads supplied in the ChargeSwitchgDNA Normalized Buccal Cell Kit in this protocol. The volumes given are on a per sample basis. Step 3: Choose a sterile cotton, foam, or flocked swab. A soft foam swab can be rubbed in the cheek pocket to secure a high-quality DNA sample. Note that the pH must be between 8.5-9.0 otherwise the DNA will not elute. 9. An official website of the United States government. 6" sterile elongated HydraFlock swab and protective cap with polystyrene handle, As test sensitivity can vary, a larger sample is generally preferred to ensure enough material is available for accurate results. What does the binary number 0111 represent? How to Collect a Buccal Swab Sample for Forensic Analysis, 3. No. 2. Add 1 ml of Lysis Mix (see above) and incubate at 37C for 20 minutes (use a heating block). The cotton-tipped swabs are placed in the subject's mouth and the inside of the cheek is vigorously swabbed, resulting in the transfer of buccal cells onto the cotton . Recommendations on Collecting and Storing Samples for Genetic Studies Due to their versatility and affordability, cotton swabs are a leading choice for many police department forensic labs. Proteins and other contaminants are not bound and are simply washed away in an aqueous wash buffer. Direct the ChargeSwitch Wash Buffer over the pellet of magnetic beads in such a way that the beads are briefly resuspended in solution. Your saliva is you, distilled into fluid form, Smith says. Dr. Jasleen Kukreja and the Life-Saving Gift of Breath, Care, Convenience and Support at New Cancer Facility, 10 Ways to Get the Most Out of Your Doctors Visit, UCSF Health Ranked Among Nation's Top 10 Hospitals. Start with 96 buccal cell samples in a 96 x 2 ml deep well plate or 96 x 2 ml filter plate. For best results, ship . Foam swabs come in several varieties to accommodate larger (adult) or smaller (pediatric) surfaces. We take the mystery out of genetics. Cotton swab derived buccal cells were scraped from the inner side of cheeks from 16 subjects, and blood was also drawn from the same 16 subjects participating in a genotypic association study of a lipid disease. When DNA is packaged in the nucleus, it is wound tightly around proteins. Looks like youre visiting UCSF Health on Internet Explorer. 2023 Jun;11(6):e2156. Add to this an appropriate transport device, and individuals performing sampling can be confident in their results accuracy. To elute nucleic acids, the charge on the surface of the bead is neutralized by raising the pH to 8.5 using a low salt elution buffer (see figure below). Why is a saline solution used for isolating cheek cells? Buccal swabs are a relatively non-invasive way to collect DNA samples for testing. One of the most common and simple ways to conduct DNA testing is with a buccal swab. How Accurate Is a Cheek Swab DNA Test? | Dynamic DNA Labs Early investors include Alphabet (Googles parent company) and WuXi Healthcare Ventures (a Chinese company). Foam-tipped swabs can range in size from small to large, being selected according to the need and purpose, in order to minimize the subject's discomfort or collect . Add 150 l of ChargeSwitch Elution Buffer (E5) (or TE Buffer, pH 8.5) to the tube and pipet up and down gently 10 times to resuspend the magnetic beads. In case of the buccal calls, the R values are very low, due to which a valid call could not be determined by the Genome Studio software. Wait for 10 seconds. There have been conflicting reports about the methodology and results obtained from using buccal DNA. With some simple training, almost anyone can collect a buccal swab sample for forensic analysis. 2019 Jan 31;14(1):e0211743. When resuspending pelleted ChargeSwitchMagnetic Beads, make sure that all beads are fully resuspended to maximize DNA recovery. Add 140 l of Purification Mix (see above; make sure that the beads are thoroughly resuspended). As with all tests, there is always the chance that you will receive incorrect results. . Clin Chem Lab Med. Editor's note: This post was originally published Aug 25, 2014, and was updated on March 9, 2023, for accuracy and comprehensiveness. The tube will have your patient label on it. Fertil Steril. In low pH conditions, the CSTbeads have a positive charge that binds the negatively charged nucleic acid backbone (see figure below). Graduated from ENSAT (national agronomic school of Toulouse) in plant sciences in 2018, I pursued a CIFRE doctorate under contract with SunAgri and INRAE in Avignon between 2019 and 2022. CS15096) is a magnetic separation rack that can hold up to 96 samples in a deep well plate. Why Is It Easy To Extract DNA From Bananas. Introduction Since 1997, select states have been collecting DNA samples from particular arrestees. You may need to perform a second elution to recover all DNA. Do not store samples at room temperature as bacteria and nucleases present in the sample will degrade the DNA. Immediately place the swab in an aerated or air permeable container, such as the Cap-Shure, FABUSA or paper wrapper, or, place the dried swab into a dry transport tube. Elution Buffer Invitrogen ChargeSwitchElution Buffer (E5; 10 mM Tris-HCl, pH 8.5) is supplied with the kit for eluting the DNA from the Invitrogen ChargeSwitchMagnetic Beads. Memorial Sloan Kettering was founded in 1884, and today is a world leader in patient care, research, and educational programs. 2014 Nov;102(5):1233-43. doi: 10.1016/j.fertnstert.2014.07.1208. Although youd be forgiven for assuming they do the same thing, the testing involved in a saliva sample is different from a buccal swab. sharing sensitive information, make sure youre on a federal (Pineapple and papaya juice perform the same function in this experiment as meat tenderizer.) 12. 2016 Oct;31(10):1711-1716. doi: 10.1111/jgh.13331. Generally with self-collection the swab may need to be slipped back into a paper wrapper or, And, if the swab is for a kit, is a single or double pack desired? There are many reasons to prefer buccal cell collection, including: Unlike other DNA collection methods, is no need to poke, prod, or draw blood to get a great sample. This site needs JavaScript to work properly. A buccal swab is the technical term for what most people would know as a cheek swab DNA test. They provide high-quality samples that can be used for PCR testing, genetic sequencing, microarray analysis, and most importantly - forensic analysis. Genotyping results can be seriously compromised if care is not taken to check the quality and yields of such specimens. Incomplete disso-ciation of DNA from the ChargeSwitch Magnetic Beads. Slowly aspirate all of the supernatant and discard, leaving behind the pellet of beads. Quantitating DNA Yield To quantitate yield of your DNA, we recommend using the Quant-iT DNA Assay Kit, High Sensitivity (Cat. Step 11: Label the tube or envelope with identifying information. Forensic Medicine Sourcebook. 7. What Is a DNA Buccal Swab Test? | Buccal Swabs Definition | Dynamic DNA Use a stopwatch to time the collection for at least 30 seconds per swab to collect cells. Why is a buccal cheek mouth sample of cells so often taken for testing? This means that womens saliva could contain evidence of unwanted attention in cases of assault, or even telltale signs of infidelity. 11021-10). We tested three types of medical-grade buccal swabs against standard cotton swabs for differences in DNA yield. However, some applications may demand a larger sample size. Move samples to the shaker. The term superfecundation is derived from fecund, meaning the ability to produce offspring. The https:// ensures that you are connecting to the Links to other sites are provided for information only -- they do not constitute endorsements of those other sites. Detroit: Omnigraphics, 1999; 45-59. If you are using the ChargeSwitchgDNA Normalized Buccal Cell Kit (Cat. Use only the ChargeSwitchMagnetic Beads supplied in the ChargeSwitchgDNA Buccal Cell Kit in this protocol. Incubate the sample at 37C for 20 minutes. 1. Shake at medium fast speed (e.g. But DNA testing isnt risk-free far from it. The tube rack can hold up to 24 microcentrifuge tubes. What is a buccal swab and what is it used for? Open the tube. ANSWER: All legal DNA tests require a consent signature from the person whose samples were submitted. Information developed by A.D.A.M., Inc. regarding tests and test results may not directly correspond with information provided by UCSF Health. for anything from ancestry DNA research to personalized nutrition. Storing DNA Store the purified DNA at -20C or use immediately for downstream applications such as PCR. Step 8: Now, repeat step 7 on the other side of the mouth with the same swab. 6th ed. PMC How do you use a buccal swab kit? What is a buccal swab used for? - Studybuff.com The way it works is that the swab collects sample cells from the inside of your cheek, which contain DNA information in the form ofbuccal epithelial cells. The health care provider will gently scrape the inside of the cheek with a small spatula to collect cells for testing. Safety Information Follow the safety guidelines below when using the ChargeSwitchgDNA Buccal Cell Kits. Use a stopwatch or the second hand of a watch/clock to time the collection. A buccal smear is a noninvasive, fast, and relatively inexpensive diagnostic method for collecting genetic material. Why are Buccal (cheek) swabs - DNA Confidential Zimbabwe - Facebook Do not freeze the beads as this irreparably damages them. The ChargeSwitch gDNA Buccal Cell Kits are designed to allow isolation of the following amounts of genomic DNA from human buccal cell swabs or pelleted cells from a mouthwash. Incubate at room temperature for 1 minute. See this image and copyright information in PMC. dealing with a small sample size and looking for a, to standard cotton or foams swab, may benefit from the use of. Retrieved August 19 from, Foam Tipped Applicator Swab with Hollow Polystyrene Shaft, Small Rectangular Foam Tipped Swab With Polypropylene Handle, DNA Free, Ultra Flocked Swab with Transport Tube, DNA Free Cotton Tipped Applicators with Dry Transport Tube, DNA Controlled Cotton Tipped Applicator with Transport Tube, http://www.hitdna.com/images/PDFs/publications/Taking%20a%20Buccal%20Swab.pdf. http://www.forensicscienceresources.com/CAPE3.htm, The Top 6 Benefits of Buying American-Made Swabs, How to Collect a Throat Swab Specimen [VIDEO], Two sterile swabs with cotton, foam, or flocked tips, Consent form (if required by jurisdiction). Note: You may vary elution volume depending on your needs. Do not freeze the beads as they will become irreparably damaged. Slowly aspirate supernatant containing the DNA to a 96 x 300 l U-bottomed microtiter plate. Another advantage of using swabs is that they allow selective collection of buccal cells, the major component of the inside of the cheek 4. Your healthcare provider or a staff member will give you a buccal (cheek) sample collection kit (see Figure 1). Our scientists pursue every aspect of cancer researchfrom exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. What is a buccal swab used for? 21. The salt, i.e. Add 150 l of ChargeSwitchElution Buffer (E5). The .gov means its official. If the swab has a breakpoint indicator on the handle, do not touch the swab above the breakpoint indicator mark. Diagnostic technicians may choose to cut the tip of the swab into a vial or to elute the sample into a buffer. Federal government websites often end in .gov or .mil. Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved. Important: The DNA yield varies and is dependent on several factors including the technique of the person taking the swab, whether the donor is a high or low shedder, and the type of swab used. Quantitating DNA Yield To quantitate yield of your DNA, use the Invitrogen Quant-iT DNA Assay Kit, High Sensitivity (Cat. Electrophoretic analysis of genomic DNA from blood and buccal cell samples DNA. The way it works is that the swab collects sample cells from the inside of your cheek, which contain DNA information in the form of buccal epithelial cells. Buccal swab samples can be stored for up to 2 weeks at 4 C before processing for DNA extraction. Push on the back end of the swab stick so the swab tip comes off. Non-invasive DNA collection methods are preferred over phlebotomy in order to increase study participation and compliance in research centers and for sick patients in hospital settings. AB-0932), 96 x 300 l U-Bottomed microtiter plate (Greiner, Cat. In this protocol, the aim is to get a sample of DNA from cheek cells. sample collection guide today! No matter how fleeting the encounter, the DNA will hang around in their mouth for at least an hour. You will need a shot glass and some ice-cold alcohol of your choice. Why is pineapple juice used in DNA extraction? Foam tipped swabs can range in size from tiny (to minimize subject discomfort) to large (for high article collection capacity). Have the following materials on hand before beginning: Before Starting Perform the following before beginning: Preparing the Lysate Follow the procedure below to prepare a lysate from the human buccal cell swab. Important: When using the ChargeSwitchgDNA Normalized Buccal Cell Kit, elute the genomic DNA in 150 l of ChargeSwitchElution Buffer (E5). Many law enforcement facilities prefer collection of buccal cells by swabbing to other sample collection methods because it is fast, non-invasive, relatively comfortable, and can yield larger sample sizes than other collection methods. 5. Search Wait for 10 seconds. Differences in AMY1 Gene Copy Numbers Derived from Blood, Buccal Cells and Saliva Using Quantitative and Droplet Digital PCR Methods: Flagging the Pitfall. NFSTC Science Serving Justice. Here are a few of our most popular swabs for buccal cell collection: Want to learn more about how to collect a buccal cell sample for forensic analysis, or which swabs to choose for your buccal swab collection kit? Why do we collect DNA from buccal samples? Please do not use it to ask about your care. Any liquid handling robotic workstation with a gripper arm, Appropriate tips for liquid dispensing and aspiration (see below for factors to consider), Incubator, heat block, or water bath for heating samples, 96 x 2 ml deep well plate(s) (Greiner, Catalog no. National Library of Medicine A small swab (like a Q-Tip) is used to scrape the inside of your cheek. 6l of DNA was loaded on a 1% agarose gel stained by GelRed(R) was visualized using a GelDoc system. Unable to load your collection due to an error, Unable to load your delegates due to an error. 23. This section provides a general protocol for automated isolation of genomic DNA from human buccal cell swabs in a 96-well format using the ChargeSwitchgDNA Buccal Cell Kit (Cat. Visit our training center, where you can view videos on a range of applications from food safety to microbiology. 18. Note that the protocol is optimized for efficient purification of DNA from small sample volumes. Buccal Swabbing is a non-invasive procedure that takes a few minutes. Yet, though the individual cells appear very simple under the microscope, they each contain the genetic make-up of the entire body. To build a custom buccal swab collection kit specific to the needs of your department, speak with your lab manager to determine the requirements or collection priorities of your lab. Cotton swabs are widely used in the field of DNA sampling, for both forensics and human genetics applications. Why? Quantitation of the cellular content of saliva and buccal swab - Nature Dynamic DNA Labs wishes you a happy 4th of July! Sex chromatin test; Buccal swab. A few of the most popular Puritan foam swabs for genetic sampling include: with tapered acetal handle features a smaller tip to minimize patient discomfort in forensics and genetics applications. Genetic characterization of the Berber-speaking population of Souss (Morocco) based on autosomal STRs. , If youre going to bite, be extremely gentle. When theres a match, investigators can undoubtedly link a suspect to the scene of a crime. Would you like email updates of new search results? Any duplication or distribution of the information contained herein is strictly prohibited. The purified genomic DNA is suitable for use in downstream applications such as PCR. Our Buccal Swab Collection Kit is the least intrusive of our series of Suspect DNA Specimen Collection Kits and is designed for use by non-medical personnel. Process samples immediately after collection or store at 4C for up to 2 weeks. A buccal cell specimen is ideal for evidence collection because it remains stable for a long time. We recommend using the 96 x 2 ml glass-filled polypropylene UnifilterMicroplate from Whatman (Catalog no. Avoid repeatedly freezing and thawing DNA. Saliva as an alternative source of high yield canine genomic DNA for genotyping studies. Other 96 x 2 ml filter plates are suitable.Before Starting Perform the following before beginning: Automated Protocol Follow this protocol to isolate genomic DNA from buccal swabs. The buccal space is part of the subcutaneous space, which is continuous from head to toe. Buccal cells are most commonly collected for DNA analysis and testing, culture experiments, and microscopic analysis. Introduction This section provides general information needed to use the ChargeSwitchgDNA Buccal Cell Kits (Cat. Nos. Place the swab tip in your mouth and rub the side of your inner cheek about 6 times. Well explain in todays blog. After incubation, transfer or filter as appropriate, as much of the lysate as possible to a 96 x 2 ml deep well plate, without interfering with the samples. Can you do a DNA test without someone knowing? From diagnosis to treatment, our experts provide the care and support you need, when you need it. Wait for 1 minute. Visit our training center, where you can view videos on a range of applications from food safety to microbiology. Wait for 60-90 seconds. 9. After saliva collection was completed, buccal swabs were collected with two Copan FLOQSwabs, Interpath, Heidelberg West, Australia), using methods that involved a member of the . 17. 11. Place the cheek brush back into the original tube. Not for use in diagnostic procedures. Product Description. 6. doi: 10.1371/journal.pone.0170767. Place the swab tip in your mouth and rub the side of your inner cheek about 6 times. doi: 10.1371/journal.pone.0211743. Below we outline the best applications for cotton, foam and flocked DNA swabs. A buccal swab, also known as buccal smear, is a way to collect DNA from the cells on the inside of a person's cheek. Quality and quantity of dromedary camel DNA sampled from whole-blood, saliva, and tail-hair. as repeated segments . 20. Illumina DNA test panel-based genotyping of whole genome amplified-DNA extracted from hair samples: performance and agreement with genotyping results from genomic DNA from buccal cells. Buccal sample swabs are generally preferred by those looking for DNA testing because theyre much less invasive than a blood test. She should look for spilled soda on the table to collect saliva. When you get your kit, you should wash your hands and find a clean flat surface to use as your work area. Do not vary the amount of ChargeSwitchElution Buffer (E5) used otherwise the yield will no longer be normalized at 1-3 ng/l. Process buccal swabs immediately after collection or store at 4C. Common Uses for Buccal Swabs All you need to do is have the subject open his/her mouth. Add 140 l of Purification Mix to the sample and pipet up and down gently 5 times to mix. Do not substitute ChargeSwitch Magnetic Beads provided in the ChargeSwitch gDNA Buccal Cell Kit for those in the ChargeSwitch gDNA Normalized Buccal Cell Kit. After incubation, transfer or filter as appropriate, as much of the lysate as possible to a 96 x 2 ml deep well plate, without interfering with the samples. Without removing the tube from the MagnaRack, carefully remove the. NFSTC Science Serving Justice. Chernecky CC, Berger BJ. IntroductionThis section provides a general protocol for automated isolation of genomic DNA from human buccal cell swabs in a 96-well format using the ChargeSwitch gDNA Normalized Buccal Cell Kit (Cat. World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonization Project: III. 15. Copyright 2023. The information on this document and/or our website is not intended or implied to be a substitute for professional medical advice, diagnosis, or treatment. The deep well plate fits onto the magnetic base station, associating the array of 24 neodymium magnets with the samples for on the magnet and off the magnet sample processing (see figures below).Deck Set Up Once you have the required hardware, you will need to configure the deck of your liquid handling robot appropriately to process samples. No test is 100 percent accurate. These sterile DNA swabs come in many different shapes and sizes. Call 911 for all medical emergencies. Make sure you lead with your lips and keep the tongue action to a minimum, at least at first. Other liquid handling robots are suitable provided that each is equipped with a gripper arm, a 96-well magnetic separator, and other additional hardware. Your saliva, after rinsing your mouth will naturally contain cheek cells, which will be broken open during the protocol to release the DNA. The cheek cells are used for DNA testing (also referred to as DNA profiling and DNA fingerprinting). What is a buccal swab for DNA? - Rampfesthudson.com This is not possible in all cases, as the swab should first address the comfort of the donor. 96-well U-bottomed microtiter plate (for final elution), To resuspend beads prior to mixing with solution, Used to mix beads or bead/DNA pellet with buffer, Normal liquid parameters for adding a reagent to each well. Without removing the tube from the MagnaRack, carefully remove the supernatant and discard. 6. Materials Needed Have the following materials on hand before beginning: Components Supplied with the Kit includes Invitrogen products such as. 11020-10), see Automated Extraction Normalized DNA Buccal Kit, This section provides a general protocol for automated isolation of genomic DNA from human buccal cell swabs in a 96-well format using the ChargeSwitch, The ChargeSwitchMagnetic Beads supplied in the ChargeSwitchgDNA Normalized Buccal Cell Kit differ in concentration from those supplied in the ChargeSwitch, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Enhanced Automated Immunomagnetic Separation (eAIMS) for Escherichia coli O157, Extraction of DNA From Plants Using Plant DNAzol Reagent, How to Use Phenol / Chloroform for DNA Purification, High Throughput Isolation Of PCR Products Using ChargeSwitch PCR Clean-Up, iPrep GeneCatcher gDNA Blood Kit - for purification of gDNA from human blood using the iPrep Purification Instrument, Isolation of Genomic DNA from Tissue Using ChargeSwitch Technology, Purification of Genomic DNA Using PureLink Silica Columns, PureLink HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA, Qualitative Verification of Target Sequence Using Platinum Taq DNA Polymerase, TRI Reagent DNA/Protein Isolation Protocol, Isolating Genomic DNA from Individual Samples, General Information - Automated Sample Processing, Automated Extraction - Normalized DNA Buccal Kit, ChargeSwitch Nucleic Acid Purification Technology, 1-3 ng/l in 150 l (Normalized Buccal Cell Kit) or up to 6g (Buccal Cell Kit), Varies (depends on quality of starting material), 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20, Uses a magnetic bead-based technology to isolate genomic DNA without the need for hazardous chemicals, centrifugation, or vacuum manifolds, Rapid and efficient purification of genomic DNA from human buccal swabs in less than 15 minutes following sample preparation and lysis, Simple lysis with Proteinase K without the need for any mechanical lysis, The purified genomic DNA demonstrates improved downstream performance in applications including PCR, Includes a kit designed for automated processing of large numbers of samples in 96-well plates using a liquid handling robot, A magnetic separation rack suitable for use with 1.5 ml microcentrifuge tubes (see below), 20 l, 200 l, and 1 ml sterile, pipette tips.